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Controlled Expansion of Mammalian Cell Populations by Reversible Immortalization
In 1996, reversible immortalization using SV40 large T antigens and the Cre/LoxP system was successfully achieved with primary human fibroblasts. The concept of reversible immortalization involves introducing an immortalizing agent, SV40 large T antigens, into primary cells, expanding the cells in the culture, and finally, efficiently removing the ... Read More»
Hirofumi Noguchi and Naoya Kobayashi
Review Article: J Biotechnol Biomater 2013, 3:158
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