ISSN: 2155-952X
Journal of Biotechnology & Biomaterials
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In Association with
Addressing challenges for expression of human proteins and multi-protein complexes using the Baculovirus expression system
8th Euro Biotechnology Congress
Arnaud Poterszman
Institut G�?漏n�?漏tique Biologie Mol�?漏culaire Cellulaire, France
Posters-Accepted Abstracts: Biotechnol Biomater
DOI:
Abstract
Many cellular processes are accomplished through the assembly of multiple proteins acting in concert to catalyze specific activities. Although technologies for production of recombinant proteins and complexes have been tremendously improved, sample preparation often remains challenging and can require considerable investments. Many of these proteins/ complexes cannot be purified from natural source and recombinant expression from eukaryotic host is often required. Current strategies for the production of protein/complexes in insect cells using the Baculovirus expression system will be discussed. Selected examples will include vector development for parallel expression/co-expression screening, use of Lambda red recombination in E. coli for manipulation and improvement of the Baculoviral genome and assembly of multi-gene constructs from synthetic biology approaches.Biography
Email: Arnaud.POTERSZMAN@igbmc.fr
