天美传媒

Abstract

Carvacrol (2-methyl-5-(1-methylethyl)-phenol) is a predominant monoterpenic phenol which occurs in many essential oils of the family Labiatae including Origanum, Satureja, Thymbra, Thymus, and Corydothymus species . It is well known for its anti-inflammatory, antioxidant and antitumor activities. But its mechanism of anti-inflammatory action is still unclear, hence in this study an attempt is made to unravel the mechanism of anti-inflammatory activity of carvacrol in D-galactosamine (D-GalN)- induced hepatotoxic rats. The mRNA and protein expression levels of tumor necrosis factor-alpha (TNF-α), interleukin-6 (IL- 6), inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and nuclear factor kappa-B (NF-κB) have been assayed by semi-quantitative reverse transcriptase polymerase chain reaction (RTPCR) and western blot analysis. We found that the mRNA and protein expressions of TNF-α, IL-6, iNOS, COX-2 and NF-κB significantly were up-regulated in D-galactosamine induced hepatotoxic rats and treatment with carvacrol significantly down-regulated the expressions of these genes showing the mechanism behind the anti-inflammatory activity of carvacrol. All above results revealed that the carvacrol well known anti- inflammatory activities in D-galactosamine induced hepatotoxic rats.

Biography

Balakrishnan Aristatile has completed his Ph.D. from Bharathiar University, Tamilnadu, India and currently working in King Saud University, Saudi Arabia. He has published several articles in reputed journals and international conferences. He is mainly working in liver toxicity .