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Proper understanding of genetic mechanisms involving in the expression of total phenolic content and antioxidant
activity would help in planning effective breeding programs in sesame. The objective of this study was to evaluate
genetic variability for total phenolic content and antioxidant capacity in twelve sesame lines and investigate gene action
in 6x6 diallel analysis. The field experiment was conducted at Mora during cropping season from 2011 to 2013 in
randomized complete block design (RCBD) with three replicates. Data analysis showed significant (p<0.05) variability
in the sesame cultivars for these traits. Broad sense heritability was high for total phenolic content (h2=0.96) and for
antioxidant activity (h2=0.99), indicating preponderance of genetic factors controlling these traits. The value of 2GCA/
2SCA ratio was less than one for total phenolic content (0.38), suggesting the prevalence of non-additive gene effects
in the genetic control for this trait, while for antioxidant activity, its value was greater than one (3.73) showing the
preponderance of additive genes effects. The parents differed for their general combining ability (GCA) and the crosses
showed specific combining ability (SCA). Dominant genes have positive effects for these traits. Genotype variation for
phenolic content and antioxidant activity indicates that, it would be possible to select for these quantitative traits in a
breeding program.