Journal of Fisheries & Livestock Production
天美传媒 Access
Our Group organises 3000+ Global Events every year across USA, Europe & Asia with support from 1000 more scientific Societies and Publishes 700+ 天美传媒 Access Journals which contains over 50000 eminent personalities, reputed scientists as editorial board members.
天美传媒 Access Journals gaining more Readers and Citations
700 Journals and 15,000,000 Readers Each Journal is getting 25,000+ Readers
Indexed In
- Index Copernicus
- Google Scholar
- Sherpa Romeo
- 天美传媒 J Gate
- Academic Keys
- Electronic Journals Library
- RefSeek
- Directory of Research Journal Indexing (DRJI)
- Hamdard University
- EBSCO A-Z
- OCLC- WorldCat
- Scholarsteer
- SWB online catalog
- Virtual Library of Biology (vifabio)
- Publons
- Euro Pub
- Cardiff University
Useful Links
Recommended Journals
Related Subjects
Share This Page
Hemoglobin from the blood clam Tegillarca granosa (Tg-HbIIA, Tg-HbIIB): Expression and antibacterial activity of recombinant proteins
6th Global Summit on Aquaculture and Fisheries 2017
Yongbo Bao and Danli Song
Zhejiang Wanli University, China
Posters & Accepted Abstracts: J Fisheries Livest Prod
DOI:
Abstract
The hemoglobins produced by Tegillarca granosa have antibacterial activity toward some Gram-positive and Gram-negative bacteria. In this study, the genes encoding the recombinant proteins Tg-HbIIA and Tg-HbIIB were cloned from T. granosa hemocytes by RT-PCR, and the proteins were expressed in Escherichia coli Transetta (DE3). The proteins were purified using a HisTrap FF affinity chromatography column under denaturing conditions and refolded at 4 �?掳C by urea gradient dialysis, and the antibacterial activity of the recombinant proteins was determined. The Tg-HbIIA protein had antibacterial activity toward Vibrio harveyi and Pseudomonas putida, with the minimum inhibition concentration (MIC) values of 6.58�?�?10-2 mg/ml and 4.11�?�?10-3 mg/ml, respectively. The Tg-HbIIB protein had antibacterial activity toward V. harveyi, P. putida and Acinetobacter baumanii, with MIC values of 1.58�?�?10-1 mg/ml, 3.95�?�?10-2 mg/ml and 7.90�?�?10-2 mg/ml, respectively. They had no antibacterial activity against Staphylococcus aureus, E. coli, Bacillus firmus, B. subtilis, S. epidermidis or V. parahaemolyticus. This study provides a basis for further research on the antibacterial function and mechanism of hemoglobin.Biography
Yongbo Bao has expertise in three field of his research: (1) Immune mechanism of molluscs: Molluscan immune and disease-resistant gene function, immune mechanism, antibacterial peptides and antibacterial mechanism etc. (2) Mollucan genetics and molecular assistant breeding: Population genetic structure, genetic linkage map, SNP and GWAS and phenotype association analysis. (3) Gene and molecular phylogeny and evolution: Immunity and development related gene function study and molecular evolution analysis.
Email: bobbao2001@gmail.com
