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PnTx2-6 recombinant protein expression using the baculovirus expression vector system
10th Asia-Pacific Biotech Congress
Seung-Won Park, Gwang-Ho Choi, Seong Ryul Kim and Tae Won Goo
Catholic University of Daegu, South Korea Dongguk University, South Korea National Academy of Agricultural Science, South Korea
Posters & Accepted Abstracts: J Biotechnol Biomater
DOI:
Abstract
Nitric oxide is the principal mediator of smooth muscle relaxation and erectile function of corpus cavernosum. A baculovirus expression vector system (BEVS) is used routinely to produce recombinant proteins in the milligram scale. PnTx2-6, a toxin from Phoneutria nigriventer spider venom, modulates voltage gated Na+ channels and activation of NO production. PnTx2-6 consists of a signal peptide, Glu-rich region and a mature toxin of 48 amino acids. To purify the recombinant PnTx2-6 protein from cells overexpressing the Phoneutria nigriventer spider venom full-length PnTx2-6 gene, recombinant baculovirus is infected and recovered in the Sf9 cells. As a result, recombinant PnTx2-6 protein was efficiently expressed and extracted from the insect cells using the BEVS. Further studies are required to whether PnTx2-6 was able to increase production rate of NO by NOS and can be released from the endothelium in certain vascular disease.Biography
Seung-Won Park has completed his PhD from Catholic University of Korea and Postdoctoral studies from Rutgers New Jersey Medical School. He is the Assistant Professor of Catholic University of Daegu, South Korea. He has published more than 25 papers in reputed journals.
Email: microsw@cu.ac.kr
